melatonin impact on in vitro development of mouse preantral follicles and oocyte maturation
نویسندگان
چکیده
introduction: melatonin acts as an indirect antioxidant and is a powerful direct free radical scavenger and direct responses to melatonin in the gonads are detected. this study aims to investigate the influence of different doses of melatonin on preantral follicle development and oogenesis of in vitro cultured mouse ovarian follicles. materials and methods: preantral follicles with diameters of 150–175 μm were mechanically isolated from nmri mouse ovaries. follicles were cultured in droplets of α-minimal essential medium (α-mem) supplemented with 5% fbs, 100 miu/ml rhfsh, 1% its, 100 iu/ml penicillin and 100 μg/ml streptomycin in conjunction with varying doses of melatonin (0, 1, 10, 100 nm and 100, 500 pm) for six days. on day six, in vitro ovulation was induced by the addition of hcg/regf to the culture medium and after 16-20 h the maturation state of the oocytes was assessed. results: there was a significant (p<0.05) decrease in the number of surviving follicles in the groups that received 10, 100 nm and 500 pm melatonin compared to the other groups. after induction of in vitro ovulation, follicles in groups that received 1, 10, and 100 nm melatonin had higher ovulation rates (p<0.05) compared with the other groups. oocyte maturation capacity was adversely influenced by five concentrations of melatonin and gv arrest was significantly higher compared to the control group (p< 0.01). conclusions: our data indicates that a dose of 100 pm melatonin has no toxic effects on follicular development and can be used to reduce oxidative stress in follicle culture systems.
منابع مشابه
Melatonin Impact on In Vitro Development of Mouse Preantral Follicles and Oocyte Maturation
Introduction: Melatonin acts as an indirect antioxidant and is a powerful direct free radical scavenger and direct responses to melatonin in the gonads are detected. This study aims to investigate the influence of different doses of melatonin on preantral follicle development and oogenesis of in vitro cultured mouse ovarian follicles. Materials and Methods: Preantral follicles with diamet...
متن کاملMelatonin Impact on In Vitro Development of Mouse Preantral Follicles and Oocyte Maturation
Introduction: In vitro environments expose developing oocytes to excess reactive oxygen species (ROS) which are not normally produced in vivo. Melatonin acts as an indirect antioxidant and is a powerful direct free radical scavenger and direct responses to melatonin in the gonads. This study aims to investigate the influence of different doses of melatonin on follicle development and oogenesis ...
متن کاملEffect of Fibroblast Co-culture on In Vitro Maturation and Fertilization of Mouse Preantral Follicles
متن کامل
Activin promotes oocyte development in ovine preantral follicles in vitro
Activins have been implicated as important regulating factors for many reproductive processes. The aim of this study was to determine the effect of activin A on the development of ovine preantral follicles in vitro. Mechanically isolated preantral follicles (161 +/- 2 microm) were cultured for 6 days in the presence of human recombinant activin A (0, 10 and 100 ng/ml). Half of the medium was re...
متن کاملEffect of BMP-6 on development and maturation of mouse preantral follicles in vitro
The aim of this study was to investigate the effect and mechanism of bone morphogenetic protein-6 (BMP-6) on the growth and maturation of mouse follicles in vitro. Preantral follicles isolated from mice were incubated with recombinant human BMP-6 (rhBMP-6) before analysis. BMP-6 expression was detected by immunofluorescence and western blot. Maturation of oocytes was observed microscopically. E...
متن کاملP-23: Effect of Melatonin on In vitro Maturation, Fertilization and Resulted Embryo Development in Mouse
Background: Melatonin has been recognised as effective reactive oxygen species (ROS) scavenger. antioxident melatonin effects has been expressed in management of cancer, alzheimer, diabet diseases. This study designed to determine the effect of melatonin on in vitro oocytes maturation and fertilization and cleavage and blastocyst development rate of mouse. Materials and Methods: Cumulus - oocyt...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
anatomical sciences journalجلد ۱۰، شماره ۱، صفحات ۷-۱۴
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023